Wavefront shaping: A versatile tool to conquer multiple scattering in multidisciplinary fields

Optical techniques offer a wide variety of applications as light-matter interactions provide extremely sensitive mechanisms to probe or treat target media. Most of these implementations rely on the usage of ballistic or quasi-ballistic photons to achieve high spatial resolution. However, the inherent scattering nature of light in biological tissues or tissue-like scattering media constitutes a critical obstacle that has restricted the penetration depth of non-scattered photons and hence limited the implementation of most optical techniques for wider applications. In addition, the components of an optical system are usually designed and manufactured for a fixed function or performance. Recent advances in wavefront shaping have demonstrated that scattering- or component-induced phase distortions can be compensated by optimizing the wavefront of the input light pattern through iteration or by conjugating the transmission matrix of the scattering medium. This offers unprecedented opportunities in many applications to achieve controllable optical delivery or detection at depths or dynamically configurable functionalities by using scattering media to substitute conventional optical components. In this article, the recent progress of wavefront shaping in multidisciplinary fields is reviewed, from optical focusing and imaging with scattering media, functionalized devices, modulation of mode coupling, and nonlinearity in multimode fiber to multimode fiber-based applications. Apart from insights into the underlying principles and recent advances in wavefront shaping implementations, practical limitations and roadmap for future development are discussed in depth. Looking back and looking forward, it is believed that wavefront shaping holds a bright future that will open new avenues for noninvasive or minimally invasive optical interactions and arbitrary control inside deep tissues. The high degree of freedom with multiple scattering will also provide unprecedented opportunities to develop novel optical devices based on a single scattering medium (generic or customized) that can outperform traditional optical components

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Influence of 3D Printed Topological Structure on Lightweight Mullite Load Bearing Board in Thermal Environment

In order to achieve the purpose of resource and energy saving in the process of producing ceramics products, the hollow lightweight load bearing board in thermal environment with topological structures was made by 3D printing. In this study, the load bearing board manufactured with different topological structures such as vertical grid, oblique square grid, and honeycomb grid was printed by direct ink writing technology using the same raw material of kaolin clay and α-Al2O3 powder. The three kinds of samples were sintered at 1450°C × 3 h. The effect of printed structures on mechanical property of load bearing board samples was investigated. Moreover, the finite element simulation was used to study the stress distribution of the load bearing board. Comparing with results obtained by three kinds of samples, honeycomb grid supported samples proved to be the most appropriate structure in various directions comprehensively

How Potential Evapotranspiration Regulates the Response of Canopy Transpiration to Soil Moisture and Leaf Area Index of the Boreal Larch Forest in China

Transpiration is a critical component of the hydrological cycle in the terrestrial forest ecosystem. However, how potential evapotranspiration regulates the response of canopy transpiration to soil moisture and leaf area index of the boreal larch forest in China has rarely been evaluated. The present study was conducted in the larch (Larix gmelinii (Rupr.) Rupr.) forest, which is a typical boreal forest in China. The canopy transpiration was measured using sap flow techniques from May to September in 2021 and simultaneously observing the meteorological variables, leaf area index (LAI) and soil moisture (SWC). The results showed that there were significant differences in canopy transpiration of Larix gmelinii among the months. The correlation and regression analysis indicated that canopy transpiration was mainly influenced by potential evapotranspiration (PET), while the effect of soil moisture on canopy transpiration was lowest compared with other environmental factors. Furthermore, our results revealed that the effect of PET on canopy transpiration was not regulated by soil moisture when soil moisture exceeded 0.2 cm3 cm−3. More importantly, under the condition of sufficient soil moisture, it was demonstrated that the response of canopy transpiration to leaf area index was limited when PET exceeded 9 mm/day. These results provide valuable implications for supporting forest management and water resource utilization in the boreal forest ecosystem under the context of global warming

Responsive characteristics of soil water regimes to rainfall events in a boreal larch forest in China: Dynamic processes and decoupling effects

Investigating the soil water dynamics in relation to rainfall events is of great importance for enhancing our understanding of eco-hydrological processes and improving soil hydrological models. However, the lack of long-term, in-situ observational data and the neglect of soil moisture decoupling have hindered the study of dynamic responses in soil water regimes to rainfall events in China’s boreal forests with permafrost. This study utilized soil water content monitoring and rainfall observational data from the Xing’an larch (Larix gmenilii) forest in Northeast China during the growing seasons of 2015–2021 to evaluate the dynamic response of soil water regimes to rainfall events. The results show a significant increase in the decoupling strength between soil water and rainfall with increasing soil depth. The thresholds of rainfall amount (RA), duration (RD), and intensity (RI) for triggering soil wetting events also increased. Soil water dynamic processes exhibited inconsistent and complex patterns in response to the hierarchical effects of RA, RD, and RI along the vertical soil profile. However, the dominant controls on soil water dynamic responses to RA, RD, and RI varied depending on specific response metrics, such as the accumulated soil water content increments (ASMI), duration time (DT), and the mean slope of the soil wetting curve (Sm). Interestingly, the dominant factor for affecting response time (RT) changed from RI to RD as soil depths increased. Importantly, the decoupling of soil moisture led to a substantial reduction in the responsiveness of these metrics of subsurface soil water to rainfall, and the role of the decoupling effect diminished with increasing soil depths. These findings highlight the complexity of the dynamic response of soil water to rainfall events and provide new insights into the relationship between soil water dynamics and rainfall features through the introduction of the decoupling of soil moisture in boreal forests

Point-of-Care and Dual-Response Detection of Hydrazine/Hypochlorite-Based on a Smart Hydrogel Sensor and Applications in Information Security and Bioimaging

A novel dual-response fluorescence probe (XBT-CN) was developed by using a fluorescence priming strategy for quantitative monitoring and visualization of hydrazine (N2H4) and hypochlorite (ClO−). With the addition of N2H4/ClO−, the cleavage reaction of C=C bond initiated by N2H4/ClO− was transformed into corresponding hydrazone and aldehyde derivatives, inducing the probe XBT-CN appeared a fluorescence “off-on” response, which was verified by DFT calculation. HRMS spectra were also conducted to confirm the sensitive mechanism of XBT-CN to N2H4 and ClO−. The probe XBT-CN had an obvious fluorescence response to N2H4 and ClO−, which caused a significant color change in unprotected eyes. In addition, the detection limits of XBT-CN for N2H4 and ClO− were 27 nM and 34 nM, respectively. Interference tests showed that other competitive analytes could hardly interfere with the detection of N2H4 and ClO− in a complex environment. In order to realize the point-of-care detection of N2H4 and ClO−, an XBT-CN@hydrogel test kit combined with a portable smartphone was developed. Furthermore, the portable test kit has been applied to the detection of N2H4 and ClO− in a real-world environment and food samples, and a series of good results have been achieved. Attractively, we demonstrated that XBT-CN@hydrogel was successfully applied as an encryption ink in the field of information security. Finally, the probe can also be used to monitor and distinguish N2H4 and ClO− in living cells, exhibiting excellent biocompatibility and low cytotoxicity

Hierarchical pulmonary target nanoparticles via inhaled administration for anticancer drug delivery

Inhalation administration, compared with intravenous administration, significantly enhances chemotherapeutic drug exposure to the lung tissue and may increase the therapeutic effect for pulmonary anticancer. However, further identification of cancer cells after lung deposition of inhaled drugs is necessary to avoid side effects on normal lung tissue and to maximize drug efficacy. Moreover, as the action site of the major drug was intracellular organelles, drug target to the specific organelle is the final key for accurate drug delivery. Here, we designed a novel multifunctional nanoparticles (MNPs) for pulmonary antitumor and the material was well-designed for hierarchical target involved lung tissue target, cancer cell target, and mitochondrial target. The biodistribution in vivo determined by UHPLC–MS/MS method was employed to verify the drug concentration overwhelmingly increasing in lung tissue through inhaled administration compared with intravenous administration. Cellular uptake assay using A549 cells proved the efficient receptor-mediated cell endocytosis. Confocal laser scanning microscopy observation showed the location of MNPs in cells was mitochondria. All results confirmed the intelligent material can progressively play hierarchical target functions, which could induce more cell apoptosis related to mitochondrial damage. It provides a smart and efficient nanocarrier platform for hierarchical targeting of pulmonary anticancer drug. So far, this kind of material for pulmonary mitochondrial-target has not been seen in other reports